Wheat (Triticum aestivum L.) is a globally important crop, accounting for 20% of the calories consumed by mankind. Whole genome sequence analysis of wheat, especially the CDS region, can speed up gene mapping, trait discovery and cloning of wheat functional genes, and facilitate wheat breeding and crop protection. The GenoBaits® Wheat Exome Capture Kit is based on the newest wheat reference genome: IWGSC RefSeq V2.1. Total 1.7M probes were designed to fit into this exome capture kit, which cover 132.83 Mbps wheat CDS regions.
The latest reference genome of wheat (IWGSC RefSeq v2.1) and annotation information (IWGSC Annotation v2.1) were used in this exome capture design to improve the accuracy of probe capture.
High CDS coverage and uniform probe sequence capture
When sequencing 10G per wheat sample, the average capture probe sequence depth is ~32x, uniformity of target region is 98% (>10%). More than 97% of the CDS regions are captured. The high coverage and deep read depth are essential for the genotyping analysis of polyploidy species, such as wheat.
Validation in a wide variety of cultivars
More than dozens of wheat cultivars were validated with this kit. In additional to SNPs, Indel and CNVs can also be detected in hexaploid and tetraploid wheat varieties.
For Discovery Service
Construction of mutagenesis population mutant libraries
Genome-wide association studies of large population
BSA or linkage analysis of segregating populations